Thus, our results should be interpreted as a demonstration that Wnt signaling is disrupted; more targeted analyses of expression and function of network components and downstream targets are required to elucidate the specific effects of Wnt signaling disruption on hematopoietic gene expression.
More targeted approaches to gene expression profiling, such as real-time quantitative PCR (qPCR), have the advantage of conceptually requiring less input material and indeed are vital in validating gene expression profiles identified and inferred from array-based global transcriptome datasets and as the molecular platform in standard nucleic acid molecular diagnostics.
A more targeted approach for multi-species comparative expression analysis is to determine how different species respond to a particular perturbation such as starvation, exposure to chemicals or a naturally occurring environmental stress.
Although widely expressed, the increased cerebellar expression of CNTN1 suggested a more targeted role than a housekeeping function.
Advances in light microscopy techniques and the use of targeted expression of neuronal and molecular markers in transgenic animals allow more elaborate circuit mapping.
Subsequent research could then focus on the establishment of more targeted and individual treatment strategies in this subgroup, as previously shown for Her2/neu expression and trastuzumab treatment in breast cancer (Pegram et al, 2000).
The goat uromodulin gene promoter efficiently targeted expression of GFP in the kidney of transgenic mice and more specifically in the epithelial cells of the TAL of Henle's loop.
More effective rescue was achieved through embryonic, compared to early postnatal expression [ 11- 13], whereas targeted expression in postmitotic neurons resulted in asymptomatic mice [ 12, 14].
RCA enabled the analysis of large, transcriptomic data sets in combination with a more targeted phosphoproteomics data set to identify mechanisms of action for this AKT inhibitor that were supported by specific RNA expression and phosphoprotein changes following GSK690693 treatment.
Lentiviral vectors are also able to carry more complex payloads than murine retroviral vectors, making it possible to deliver expression cassettes that direct either constitutive or targeted expression in various hematopoietic stem cell progeny.
