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Multiplex polymerase chain reaction is a variant of PCR in which two or more target sequences can be simultaneously amplified.
Until now, the molecular mechanism of RovA-dependent transcription of the target genes has only been determined for two genes, inv and rovA, and the analysis of more target sequences will obviously be required to fully characterize a RovA binding site.
The 21 bp sequence matches one or more target sequences for cleavage [ 10, 13].
We believe that as more target sequences become available, our predicted false negative rates will tend to increase for a given published signature both as a result of better sampling of diversity and as a result of failure to detect newly evolved variants.
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I wonder whether the authors can incorporate more 5' and 3' target sequences into the pREFLECT vector to better test the efficiency at different local mRNA secondary structures.
This newly described target sequence should be preferred to less repeated or more divergent target sequences in order to improve the sensitivity of the PCR tests for the diagnosis of toxoplasmosis.
This newly described 529-bp repeat element should be preferred to less repeated or more divergent target sequences in order to improve the sensitivity of PCR tests for the diagnosis of toxoplasmosis.
The higher degree of the primer degeneracy and the low annealing temperature allow the LAD primer to bind to the target sequence with a higher probability, thus more target LTR sequence regions were efficiently created [ 23].
The higher and more constant target sequence efficiencies for Sumatran West Alas individuals (median 97%) compared to Bornean South Kinabatangan individuals (median 86%) were most likely caused by carrying out the initial in-silico digest, which predicted our target sites, on the Sumatran reference genome.
However, we have observed that the introduction of Genome Sequencer primer handle sequences (denoted A, B) can result in the formation of short unspecific products; fragments that amplify more readily than target sequences and thus threaten to severely reduce the frequencies of target sequence reads in a sample.
To address this question, we ordered two more siRNA targeting sequences for MAF1 and POLR3A.
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