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For these reasons, Pichia is a more suitable expression system for producing eukaryotic IMP than E. coli.
The tissue-specific selection of reference genes using microarray data carried out in similar conditions allows to identify novel genes having higher expression stability and a more suitable expression range than commonly used reference genes.
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Mammals seem to be more suitable as expression systems for human proteins, however, especially for those that are glycosylated.
Removal of flexible tails or separation of fragments would yield more compact and stable protein fragments that are more suitable for expression of a soluble recombinant protein and subsequent studies including crystallization, as demonstrated by data presented below.
"Because CG will be more suitable for photoreal expression, I think that CG and cameras will become more familiar, and more various expressions will be born as AR," he explains.
The nuclear medicine imaging technique would be much more suitable for detecting expression of receptors in the tumor or receptor/ligand interaction using the tagging of radioactive isotopes to the ligands.
pACYCDuet- lipA- lipB was more suitable for the expression of active LipA than pET28a- lipAB and pETDuet- lipA- lipB.
We switched to the pTRA vector series (derivatives of pPAM [ 21]) because they have a smaller backbone than pBin vectors (<3 kb) and are therefore more suitable for tandem expression cassettes.
The reason was that the PRARE2 plasmid was carried in Rosetta bacteria, which could complement tRNA corresponding to the seven rare codons absent from E. coli, and when compared with the Origami B (DE3) bacteria, it was more suitable for the expression of proteins containing a rare codon [ 19, 20].
All these scenarios clearly underscore the pressing need of a yeast expression system that is more suitable for cloning and expression of the protein of interest extracellularly by its secretory pathway.
On the other hand, 'signal log ratio' is provided with the "Differential Expression" query page, and since it is generally used for pair-wise comparisons it is more suitable to assess differential expression between monozygotic twins.
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