While this might seem obvious, more subtle views of biological plausibility may not.
However there were more subtle changes to biological processes that were not apparent during the classical physiological evaluation but were detected by global transcriptomic analysis.
As we expected to find fewer, more subtle differences in our biological model compared to previous cancer epigenomic studies, we generated our DMP lists using an uncorrected p value and then identified similarities in the lists between contrasts as an additional level of statistical robustness.
On many occasions, a reduced fragment of microarray data could work more efficiently to reveal more subtle insights into the target biological phenomena than the non-reduced global genome data do [ 22- 24].
Although many of our transcript-level changes were greater than 1.5-fold, our use of sample replicates, quality controls, and careful statistical analysis allowed us to see more subtle changes that may have biological relevance.
We investigated whether this difference could be explained by the counting algorithm, rather than being due to some more subtle difference between experimental protocols or biological conditions.
Actually, this isn't just your teenagers being difficult, for a subtle biological shift in sleep patterns occurs during puberty, probably to ensure more sleep during rapid growth.
