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In general, proteins consist of one or more structural domains.
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This could lead to either two or more smaller structural domains or one larger than average single domain.
However, as there are many more supercoiling domains than structural domains, the factors that define supercoiling boundaries must be distinct from those that characterise structural attachments.
hMAT2B superimposes well with the sugar-modifying enzymes in the core Rossmann domain, but displays more structural differences in the second domain pocket, defined by loop C (residues 282 289), the parallel β-sheet preceding it (part of loop B; residues 210 214 and 275 279), as well as the loop preceding helix αB (loop A; residues 199 206).
Therefore, edited C's outside the structural domains will more easily lose the ability to be edited, as changes in the amino acid composition will have a smaller impact on protein function.
The X-linked inhibitor of apoptosis, XIAP, is a member of a large family of proteins which share in common one or more structural motifs known as BIR domains (LaCasse et al, 1998; Liston et al, 2003).
The shrinking size of the extracted conserved region pair is not the inability of MOTIF-EM, but is due to the fact that more and more structural information is lost, especially near domain boundary, as the map resolution worsens.
Therefore, more structural studies and mutational analysis of complexes built of those domains are necessary to define the motifs and interacting residues involved.
One of the more novel findings of the epitope mapping is that substitutions in different structural domains of adjacent E-protein monomers can act as epitope determinates for a single antibody suggesting that some antibodies might recognize inter-monomer epitopes including the disparate structural domains EDII and EDIII.
5) Structural domains are defined geometrically by structural compactness, presence of a hydrophobic core and more extensive amino acid interactions intra-domain rather than inter-domain.
"The concern is more structural.
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