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Significantly, cortactin immuno-precipitated from extracts of agrin-treated myotubes was more strongly stained by the anti-phosphotyrosine antibody than cortactin captured from extracts of control myotubes ("IB: PY", lower blot).
At HH24, B-RAF was abundantly expressed in the macula sacculi (Figure 2 a d) and it was also expressed in the AVG, where a subset of neuroblasts was more strongly stained for B-RAF (Figure 2 e h).
Arrows in Figure 1D indicate that the area of the canalicular membrane was more strongly stained.
However, after 6 days of T3 treatment, clusters of cells in the epithelium appeared and were more strongly stained by MGPY than the surrounding cells (Fig. 1C).
When the mice were birthed and growing, the positive staining cells were bigger and more strongly stained in external granular layer of the cerebral cortex.
In addition, Drp1 activation differed between mitochondria expressing diverse levels of IF1, as extracts of −IF1 cells were more strongly stained with the anti-pospho-Drp1 S616 anti-pospho-Drp1 S616 anti-pospho-Drp1 S616f the protein.
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Tumours invading the bladder muscle were significantly more likely to be strongly stained positively for p53 and/or EGFr compared with superficial tumours: only 15% of invasive tumours were stained negatively for both p53 and EGFr.
At 14 DPI, light microscopy images of coronal sections evidenced striatal regions stained with blue in control animals but there were more extensive and more strongly-stained regions in GA-injected animals.
Staining was considered positive for p53 when more than 10% of the cells' nuclei were strongly stained.
Staining was considered positive for TP or VEGF when more than 10% of the tumour cells were strongly stained.
C-Kit expression was scored as negative when there was no staining for c-Kit or when staining was observed in less than 1% of the cells and as positive when the cytoplasm was strongly stained with or without membrane staining in 10% or more cancer cells.
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