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A more stringently selected cohort may have been more responsive.
In this more stringently selected data set [eight twin pairs; average 2.1 years after diagnosis (range: 0 4 years)] reflecting more the consequences of disease, we found 5188 bcDMPs, with similar directional distribution (81.7% hypo- and 18.2% hypermethylated sites) as detected before (Wilcoxon signed rank test; P < 0.05).
This pragmatic approach captures the exact context in which a diagnostic biomarker would be used by clinicians (i.e., patients with a clinical suspicion of bacterial infection), one that may lead to more generalizable results as compared with a study based on more stringently selected patients.
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We chose to calculate two separate datasets using SAM (one not stringent, and one stringent) to circumvent the issues that a stringently ascertained dataset might miss relevant pathways (because too few genes are entered in the analysis) or that a larger, less stringently selected set of genes would contain too many false positives.
Those patients had been stringently selected for trastuzumab treatment primarily by FISH (90%).
Thus, even in our stringently selected fibroblast samples and gene set, topology remains evident in the gene expression profile.
Importantly, the master regulator PPARγ was among these 26 stringently selected top ranking candidates, serving as a positive control.
The genes spotted on the array have been stringently selected from Atlantic salmon and rainbow trout expressed sequence tag (EST) databases.
Here, we use a stringently selected set of subjects to report suggestive association of SNPs in GH1 with fetal growth restriction.
The presence of these four known partners among the 89 stringently selected ('Materials and methods') and reproducibly pulled down proteins confirmed the validity of our approach.
These lncRNA transcripts were stringently selected as spliced transcripts generated from independent promoters, with limited coding potential and a codon bias characteristic of noncoding sequences.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com