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"And if the law had been more stringent, we would follow those rules".
To make the comparison more stringent, we removed distances of intra-molecular cross-links calculated on residues pairs that were less than 50 residues apart in the sequence.
To make the alignment more stringent, we used two criteria: (a) maximum of two mismatches were in the seed region of the reads and maximum sum of mismatch quality across alignment is less than 70 (b) disallowing all the reads that map at multiple sites in the reference genome.
Moreover, in order to make the filtering condition more stringent, we also require that the set above is such that for any pair of q-hits h = (i, j) and h' = (i', j') in S, the following properties hold: (2)|diag(h ) - diag(h' )| ≤ d (3) i ≠ i' (4) j ≠ j' (5) i < i' if and only if j < j' In Figure 1 we can see examples for these properties that we motivate as follows.
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The authors did not report sensitivity analysis to their accuracy threshold, and so we cannot compare findings at our more stringent, and we believe more clinically appropriate, threshold of ± 5%.
However, with a more stringent threshold, we could have missed some interesting findings.
By applying more stringent criteria, we further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR.
In this present study, protein identification is more stringent and we used the probabilistic approach of decoy database searching [40].
To further test this under more stringent conditions, we seeded wt and Sei1-null cells at low cellular density and checked for the appearance of colonies.
Using these more stringent criteria, we have elucidated additional roles for sAC in neuronal responses to the guidance cue Netrin-1 [23] and in cellular responses to the neurotrophin, Nerve Growth Factor (NGF) [27], [28].
If differences in RMR among treatment groups are not specific to such more stringent conditions, we predicted that individuals from LH and HL treatments have elevated metabolic rates as adults, compared to individuals from the HH treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com