Sentence examples for more stringent wash from inspiring English sources

We did not analyze the pool of mRNA that remained bound to Ago2 following this more stringent wash.

The mAb resin was mixed with the soluble fraction of the whole cell extract, washed with lysis buffer followed by a more stringent wash with high salt buffer, and protein was eluted by SDS sample buffer and heating.

The automated protocol included a more stringent wash routine of the immobilization beads, compared to the first sequencing library preparations to make sure that all non-immobilized DNA were removed.

The hybridization procedure was done according to the Agilent protocols, except for a more stringent wash (0.1 × SSC at 35°C for 10 minutes for the Oligo Array).

Under the circumstances, specific steps were taken to minimize this unwanted situation and may include longer antibody incubation time (overnight at 4°C) followed by more stringent wash (twice in lysis buffer and three times in HEPES buffer).

The superior strength of the biotin avidin interaction allows one to employ more stringent washing conditions in the ChIP protocol, resulting in a better signal/noise ratio.

Arrays were washed twice at 42°C with 2× SSC +0.2% SDS, and twice at 23°C with 2× SSC, followed by more stringent washes at 23°C with 0.2× SSC and with filtrated H2O.

Instead of modifying the conditions in which the selection is performed (more stringent washes in the case of phage display) we used a panel of GFPi vectors exhibiting different susceptibility to test protein folding interference (Fig. 4).

Biotinylation of rMSP142 permitted a modified MSP142 ELISA (i.e. assay 4, table 1) whereby high affinity binding to streptavidin followed by a more stringent washing protocol allowed removal of non-specific binding of human IgG.

More stringent washing conditions were used in the additional rounds of panning.

For RNA blots hybridized with longer probes, more stringent washing conditions with 0.2 x SSC, 0.1% SDS at + 65°C were applied, leading to increased specificity judged by simpler band patterns.