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Changing this threshold to more stringent values (i.e. 20 and 10 μM) did not change our results (Supplementary Table S6).
Therefore, transcripts with a tpm threshold < 0.8 were eliminated, resulting in more stringent values, coming up with 40,221 unique sequences.
It is worth noting that this value is among the more stringent values used in previous studies (Martin-Subero et al, 2009; Richter et al, 2009; Ang et al, 2010).
Note that we have chosen a rather high FDR, but the main conclusions of our comparison also hold for more stringent values (see also Additional file 1 - Figure S1).
More stringent values caused disconnection of the most diverged sequences, while more permissive values caused over-compaction of the whole dataset into a single cluster with only a few outliers.
DePristo et al. (2006) used the default values for SEG (Wootton and Federhen 1993), whereas we chose more stringent values for our analysis as indicated by the reduced number of LCR detected.
Similar(52)
Believe it or not, the new regulations actually demand that banks start doing an additional, more stringent value-at-risk test.
In total, 339 predictions showed a more stringent value of p ≥ 0.9.
Using a more stringent value of D' = 0.9 results in 2,906 more "independent" SNPs on chromosome 1 which extrapolates to 63,932 more "independent" SNPs across the genome.
Hence, to determine the sensitivity of ABS-Scan, a cut-off of 0.5, which is a more stringent value, is chosen.
The maximum expected value for BLAST (BLAST E) was changed from the default setting of 5e-3 to a more stringent value of 5e-15 in order to eliminate the possibility of false positives.
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more fundamental values
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