Importantly, the more stringent selection strategy (with less than 2% of the cells being selected in rounds 3, 4, and 5) turned out to be more efficient for enrichment of pH-dependent binders (Table 1).
Because of the smaller genome size of viruses, we used a more stringent alignment strategy (i.e., 100% identity and ≥1% coverage), and 3 most abundant viral species were identified.
In order to investigate whether a more stringent selection strategy is beneficial, two selection campaigns were performed in parallel in rounds 4, 5, and 6: either 1 2% (stringent) or 5 8% (non-stringent) of the population were selected (Supporting information, Fig. S1).
Some researchers noted the high actual FDR of novel peptides and therefore employed more stringent filtering strategies, e.g. post error probability (Brosch et al., 2011) or separate FDRs for annotated and novel peptides (Branca et al., 2014).
A great number of financial supports and a strong commitment of fish farming industries would be needed if more stringent control strategies would be applied to prevent the disease spreading northward and to minimize the number of PD outbreaks in the endemic area.
Therefore, more stringent regulations and strategies are being introduced in order to facilitate appropriate allocation of land and efficient use of land, in order to produce both food and fuels [6],[7].
Numerical examples show that the switching control strategy can cope with more stringent design specifications than the fixed gain strategy, being useful to improve the performance of this class of systems.
Our selection strategy was more stringent.
Also, new postexposure treatment approaches, based on small interfering RNA (4 ) and antisense oligomers (5, 6 ), have shown promising results in rodent models, but no reports have been published of evaluations of either strategy in the more stringent macaque models.
Following the failure of diplomatic efforts to date, the US is considering options for a "plan B" strategy, said to include more stringent sanctions against Russian and Syrian institutions and individuals.
