There will be higher levels of surveillance, more stringent searches.
Here, and in contrast, using separately-harvested bovine GV oocyte and cumulus cells, we did a separate proteomics experiment but this time using an updated bovine proteome, more stringent search criteria, and much better structural and functional annotation.
This generated scepticism about possible sequence assembly and annotation mistakes and prompted a more stringent search criterion, detailed below.
In a more stringent search, we selected only the preleukemic CGIs that were covered by at least two hypermethylated probes.
To perform more stringent search of gene gain, BLASTP searches were done on the proteins which did not show any orthologs in other three species.
In addition, these two genes were regarded as non-miR genes when we used more stringent search criteria (see supplementary table S4, Supplementary Material online).
To perform a more stringent search, however, we chose to decrease the Expectation Value (E value) from the default 5e−3 to 1e−15 to minimize false positives.
It should be noted that several miR genes were regarded as non-miR genes when we used more stringent search criteria (table 1).
Allergen Atlas allows for more stringent searches of the FAO/WHO protocol by allowing users to define input parameters such as the number of contiguous amino acids for screening (rule 1) as well as the sequence identity threshold (rule 2).
In cancer proteomics field, proteogenomics has been applied to identify SNVs that result in single amino acid variations SAVs), by constructing specialized database containing SAVs and developing more stringent database searching algorithm [ 4, 5].
