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Here, and in contrast, using separately-harvested bovine GV oocyte and cumulus cells, we did a separate proteomics experiment but this time using an updated bovine proteome, more stringent search criteria, and much better structural and functional annotation.
This generated scepticism about possible sequence assembly and annotation mistakes and prompted a more stringent search criterion, detailed below.
In a more stringent search, we selected only the preleukemic CGIs that were covered by at least two hypermethylated probes.
In addition, these two genes were regarded as non-miR genes when we used more stringent search criteria (see supplementary table S4, Supplementary Material online).
To perform a more stringent search, however, we chose to decrease the Expectation Value (E value) from the default 5e−3 to 1e−15 to minimize false positives.
It should be noted that several miR genes were regarded as non-miR genes when we used more stringent search criteria (table 1).
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There will be higher levels of surveillance, more stringent searches.
The club must also introduce more stringent searches and ID checks.
In our study, we elected to limit false negatives and identify more potential lineage-specific genes in A. thaliana by using the entire A. thaliana predicted protein-coding gene complement in our analyses and by using more stringent searching criteria.
Allergen Atlas allows for more stringent searches of the FAO/WHO protocol by allowing users to define input parameters such as the number of contiguous amino acids for screening (rule 1) as well as the sequence identity threshold (rule 2).
As the restrictions by governments worldwide on lowering of sulfur content in transportation fuels are getting more stringent, the search for alternatives to conventional hydrodesulfurization process is attracting numerous studies on desulfurization.
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