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We believe these constitute the highest-affinity interactors, given that they can endure a more stringent purification.
To address these issues, double His-tagged fusion proteins permitting more stringent purification conditions were introduced [ 33, 36].
More stringent purification and equipment cleaning procedures as well as routine testing for contaminating activity have been put in place at PBL in part due to these experiments.
For the more stringent purification presented in Figure 1 figure supplement 1 the second wash step was altered to 20 ml buffer containing 50 mM imidazole.
We also note that Pabst et al. (2002) used a more stringent purification protocol (Mono-Q purification) even though they used a different SNAP25 construct for the preparation of the SNARE complexes for their ITC studies and report Kd values similar to our findings (Pabst et al., 2002).
Similar(55)
Accompanying increases in unwanted (isobaric) contamination places more stringent requirements on beam purification, especially mass separation.
Some states are more stringent.
More stringent corporate governance rules.
They should be more stringent".
They may also establish more stringent requirements.
Individual departments may dictate more stringent requirements.
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