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While more stringent mapping quality filters can reduce these false-mapping rates, these filters would reduce the sensitivity of each of the other methods.
In addition, more stringent mapping parameters in the mapping software, e.g., the number of mismatched bases and the number of gaps, should be applied if reads from heterozygous genomes are used for SNP discovery with the AGSNP pipeline.
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Similar performance results were obtained using the more stringent exonic mapping (i.e. requiring at least one exon to be overlapped by a CNV).
However, we applied an even more stringent cutoff mapping quality scores (MAPQ > 5) and obtained ~5.5 million for IgG and ~8 million reads for LRH-1 enriched samples which were used for further analysis (Table 1 and Figure 1A).
Therefore, we took relatively stringent mapping conditions described above.
If we apply more stringent criteria to map BESs onto the Oj genome, more wild rice-specific genes that have the potential to genetically improve modern cultivated rice may be identified.
As an initial check, we modeled synthetic data using both default and more stringent convergence parameters; the maps were very similar and simple covariates were adequately controlled [Webster et al. submitted].
Currently, the scalability of this web service is that of the relational backbone - which is indeed more stringent than, for example, a map-reduction store.
This approach leads to some loss of reads that map with one end to the UTR of a gene where UTRs are not yet annotated; however, the more stringent counting also prevents spuriously mapped reads from being counted.
In his final two years in office, Bloomberg made resiliency the primary focus of the city's climate change planning, redrawing flood zone maps and imposing more stringent building codes.
For further analyzing the 134,204 well mapped G1 FLNC reads, more stringent criteria were adopted during finding their genomic loci in CS draft genome sequence using GMAP.
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