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Performance generally decreased with more stringent cutoffs (Supplementary Fig. 26a,b) although some biclustering methods and direct NI methods remained more stable across a wide range of cutoff values (Supplementary Fig. 26c,d).
When the items have different frequency distributions, Tabachnick and Fidell (2007) follow Comrey and Lee (1992) in suggesting using more stringent cutoffs going from 0.32 (poor), 0.45 (fair), 0.55 (good), 0.63 (very good), or 0.71 (excellent).
Pathway analysis of gene lists obtained using more stringent cutoffs (51% present and a 1.25-fold change) highlighted many of the same pathways related to transcriptional regulation even though the gene lists were much shorter (72 compared to 593 genes in the list of overlapping down-regulated genes) (table S4).
Author's response: Using more stringent cutoffs can eliminate false positives.
I would suggest that more stringent cutoffs be applied at all stages of the analysis.
Lower FDRs can be obtained by using more stringent cutoffs (Table 2).
Similar(41)
d f, Same as a c but using a more stringent cutoff of 99%.
Patrick J. Sullivan, the Manhattan borough president's appointee to the Panel for Educational Policy, applauded the revised standard, saying the more stringent cutoff would have shut too many students out.
c, Distribution of serotypes by year comparing the detected symptomatic infections by PCR and the augmented primary infections using a more stringent cutoff that >75% of model iterations infer the same serotype.
Assessing the relative strength, rigor, or difficulty of various policies as implemented in various states (e.g., a more lenient versus more stringent cutoff score for passing a mandatory vision test) was beyond the scope of this study.
With this more stringent cutoff (fold change >1.4), 64 genes were identified (Table 1).
More suggestions(15)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com