We attributed this increased cytotoxicity of NU1025/C-1305 combinatoon to a partial re-activation of wt p53 pathway in drug-treated HeLa cells and a more stringent cell cycle checkpoint control during G2/M transition that was associated with the enhanced cell death by mitotic catastrophe [48].
To assess the specificity of our gRNAs, we amplified and sequenced the 14 potential off-target sites defined by the more stringent cell culture-based, or "maximum", criteria and adjacent to a canonical PAM for gRNAs R2, R3, S1, and S2 in 23 independent F1 flies and observed intact sequence in every case.
For example, more stringent cancer cell specific promoters have been utilized to control E1A expression for improved specificity of CRAD replication in cancer cells [5] [7].
In vivo models are far more stringent than cell culture-based assays for demonstrating drug activity, particularly in the case of orally delivered drugs as they must be absorbed by the gastrointestinal tract without degradation, enter the blood and circulate with sufficient half-lives, and penetrate solid target organs at concentrations high enough to be potently efficacious.
The severity of CDI symptoms was assessed at baseline by research nurses using the guidelines proposed by Public Health England [ 29], which we adjusted to incorporate a more stringent white blood cell count cutoff of >20 × 10/L while also replacing acute rising creatinine with an estimated glomerular filtration rate of <30 mL/min/1.73 m at the time of diagnosis.
This clinical behaviour is likely to reveal a ER related relationship between tumour cells and the breast tissue, which may play a dominant regulatory role upon ERP breast tumour cells with more stringent constraints than upon ERN cells, for which the time to overt recurrence is similar to the corresponding time of the other tissues.
Integration of these pieces of information will allow for a more stringent control over the cell grafts and elucidate the mechanisms of the action of stem cells therapy in cerebral ischemia.
"The definition needs to be very clear, and security forces need to be able to conduct more stringent operations against such cells, the tracking and hunting down of sleeper cells needs to be dramatically improved," he said.
Cellular uptake of the nucleoside analog BrdU during S-Phase transition would, unlike DNA content, likely be more stringent in labeling proliferating cells, but the technique does not label G2/M-Phase cells, and has further limitations.
The United States did not follow suit, but it did impose more stringent constraints on mercury-cell units during the early 1970's.
Thus, the tsBN67 rescue assay is more stringent than the HeLa-cell G1-to-S progression assay because single-copy Domain 1 and 2 constructs are relatively less active in the tsBN67 rescue assay than the HeLa-cell G1-to-S phase progression assay.
