Sentence examples for more stringent alignment from inspiring English sources

An additional more stringent alignment was constructed by removing ambiguously aligned sites using Gblocks [ 69] as well as visual examination.

By re-running TopHat with more stringent alignment parameters, the percent of confirmed junctions was boosted to 94.8%, but this resulted in a 71% decrease in the number of found junctions (885).

Mode 0 is similar to the FLASH approach, but with more stringent alignment score parameters.

Because of the smaller genome size of viruses, we used a more stringent alignment strategy (i.e., 100% identity and ≥1% coverage), and 3 most abundant viral species were identified.

Hence, the more stringent the alignment parameters, the fewer usable transcripts that are obtained, leading to a decrease in performance of L_RNA_scaffolder.

More stringent post-alignment filters can be used to reduce the false-mapping rates of all methods further, but will result in some decrease in sensitivity.

As majority of the third codon sites are synonymous, we decide to set more stringent thresholds using the alignment of coding sequences without using third codons.

To make the alignment more stringent, we used two criteria: (a) maximum of two mismatches were in the seed region of the reads and maximum sum of mismatch quality across alignment is less than 70 (b) disallowing all the reads that map at multiple sites in the reference genome.

These issues could potentially be addressed by modifying BWA's parameters to be more stringent for accepting global alignments and less stringent for soft-clipped local alignments.

Finally, we also produced species-level alignments with more stringent rubrics for gene inclusion: one requiring genes to be sampled for two or more bee families (10 genes, 1336 taxa, 11944 sites, 78.4% missing), and one requiring genes to be sampled for at least four bee families (7 genes, 1328 taxa, 8467 sites, 71.7% missing).

We explored further the robustness of the phylogenetic signal of the datasets against a) the alignment deriving after highly variable regions were removed with even more stringent criteria by Gblocks and b) the alignment deriving from the default settings in ClustalX.