Based on these previous studies and to ensure the lowest rate of false positive predictions, the present study applied more strict conditions to include direct connections with known resistance genes and interactions with over four known resistance genes.
In view of these limitations, we applied more strict inclusion criteria in our study, focusing mainly on a more homogenous and relevant population.
In a recent study applying more strict diagnostic criteria for arterial dissection in children, the MCA was involved in only 22% of anterior circulation dissections (Rafay et al., 2006).
The differences used in this study are more strict than the 20% difference in the International Organization for Standardization (ISO) standard (15), or the 15% difference or a difference of 1 mmol/L in cases when the glucose concentration is <6 mmol/L in the Dutch guideline (16).
In recent studies, a more strict characterization of Treg cells has been achieved [ 23].
By contrast, our study applied a more strict inclusion criterion in case and control experimental design.
Finally, compared with Lang's study, we applied more strict inclusion or exclusion criteria, which could affect the assessment results.
Four studies (4/8) enacted more strict criteria, requiring other abnormalities in addition to positive CSF culture, such as elevated CSF white cell counts, elevated CSF protein, or clinical manifestations [ 9, 12, 27].
Although other clinical studies, with a more strict selection of patients, have been conducted, it is still not clear if the expression of EGFr is necessary for response, or whether its inhibition will represent a clinical benefit of treatment in NSCLC.
In one study glucose control was more strict in the EPN than in the placebo group (p = 0.0392) [11].
