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At this stage, more strict parameters (-minmatch 40 -minscore 60) of phrap were used to distinguish individual members of possible multi-member gene families.
ESTs that aligned to the same mRNA were assembled along with the mRNA using the phrap program with more strict parameters (-minmatch 40 -minscore 60) to distinguish possible individual members of possible multi-gene families.
To evaluate stringency parameter sets for SNP prediction and the feasibility of this SNP discovery approach for fine mapping,, we deliberately selected predicted SNPs for validation from the list of 39,022 putative SNPs predicted on the 6.5x scaffold assembly, and then applied more strict parameters for SNP calling to see how the stringency setting would affect the validation rate.
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Prophage Finder was first tested with default parameters and second with a more strict E value threshold (1e-3).
Stricter parameters help avoid nonspecific mapping, while less strict parameters allow reads with RNA editing and sequencing errors to be more readily mapped.
The more strict the definition of similarity, the lower the variability and thus the clearer the effect on ADME-Tox parameters, but also the less data pairs will be available [1].
More strict criteria were set for SSR detection with a minimum length of 18 bp for di- to hexanucleotide motifs, and the parameters for primer design were the same as before except that the PCR product size was 100-400 bp.
They must conform to very strict parameters.
"Sam's strict parameters probably played a big part.
Until then, the collection had evolved with no strict parameters, she said.
Rediscovering maverick vegetables that defy the strict parameters of EU guidelines is a joy.
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