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To extensively analyze the molecular regulation of pollen development and germination, we further screened transcripts expressed preferentially in pollen (one or more stage) using sporophytic tissues as controls (ratio ≥ 2.0, Z-score ≥ 3.19) and those shared across all pollen stages and sporophytic tissues (see Materials and Methods).
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However, X-aptamers use a pseudorandom process wherein the variable region is built in 10 or mores stages, using a split and pool method in which only a small number of DNA fragments are allowed at any one stage (Table S1 of the Supporting Information).
Then we proceed with the goal softening searching procedure in the second stage using more refined approximate models to search for a good enough solution from the subset obtained in the first stage.
This evidence could explain the fact that we have not detected any Hb9 expression in our differentiation protocol at more mature stages using 8 weeks old hNPs to generate PNS neurons.
The only use of 3D modelling was therefore in the first step of the protocol, in which a volume-rendered model of the entire embryo was used to define more precisely the embryo developmental stage, using the criteria of external features proposed by Theiler (Theiler, 1989).
Another mouse preclinical screening trial targeted the symptomatic stage (between 9 and 11 months) [ 44] and a study with a plant extract [ 99] used 23- to 24-month-old mice, but to our knowledge no chemically defined AD drug candidate has been tried at the more pathologically advanced stage used in this study.
The second stage used more detailed assessments to characterise problems.
It is observed that, if three or more stages are used, then special care should be taken in designing the STF of the third and the latter stages, which operate in the denominator of the noise-shaping term.
Festival events will be held on the Bolshoi's smaller new stage, used for more experimental productions.
That gave him even more stage time.
We therefore studied young leaf stages in more detail using the analysis tool TrichEratops [ 66].
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