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A more stable preparation at neutral pH needs to be studied so as to reduce the transport and storage costs, which should be taken into consideration for the future clinical application.
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As intravenous iron therapies have different physico-chemical properties, the administration may require more frequent lower individual doses (e.g. intravenous iron sucrose or gluconate) whereas more stable preparations (e.g. iron dextran or ferric carboxymaltose) can be given as large single intravenous doses.
Concurrent with improving the process design, development of a more stable biocatalyst preparation would be an alternative strategy.
The preparation is more stable at higher temperatures as compared to the free enzymes.
In this era of developing genetic therapeutics, there is a call for the collaborative work by academicians and industry groups in order to develop methods for preparation of more stable polymeric nanoparticles/DNA complexes and robust analytical methods to characterize formulations during formation and storage.
Four different mutants of penicillin G acylase from E. coli (PGA) were site-direct immobilized and rigidification on Eupergit C. The resulting immobilized preparations were more stable against both temperature and organic solvents than those obtained by using a more complex tailor-made disulfide-epoxy supports.
Efforts have been focused on the development of low-cost enzyme preparations and more stable and active biocatalysts, in order to improve conversion yields in a shorter period of time and to recycle the enzyme for as much batches as possible [ 13].
Immobilization of NOX onto agarose activated with glyoxyl groups yielded the most stable enzyme preparation (6-fold more stable than the hyperactivated soluble enzyme).
Furthermore, this immobilized preparation was markedly more stable than non-covalently immobilized TvDAO or the free enzyme in presence of the surfactant Pluronic F-68.
The MCF-based preparations were notably more stable than a native enzyme at 333 K and they also showed good storage stability at 277 K.
As a rule, synaptic components are more dynamic in younger preparations and become more stable in older ones.
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