Of interest, the greatest age-related changes were observed for nonimprinted loci, whereas imprinted loci appeared more stable.
Following AR activation, nucleosomes with altered H3K4me2 marks become destabilized at AR binding sites and are comparably more stable at the two flanking loci.
We observed that a k-means algorithm produced more stable clustering results on the locus link component matrix with dimensions (361 × 12) obtained from PARAFAC model.
This inter-individual difference could be reflected by the variance parameters, which showed that some CpGs could harbor wider inter-individual differences than other loci which are more stable in their methylation among the population (Fig. 1B and Supplementary Material, Table S1).
Many would acknowledge that simultaneous modeling of all loci potentially yields fairer estimates of genetic effect, more stable phenotypic predictions, and better characterization of between-locus confounding [Hoggart et al., 2008; Lee et al., 2008].
Using the iterative projection method, and in order to limit error propagation, the projection process started with the map that presents the more stable framework and well ordered and spread loci i. e. the map derived from the DYDH population.
It was our intent that assessment of CpG island methylation (as opposed to the individual locus approach) would provide more stable estimates that are less readily influenced by outliers due to technical variation.
SNPs have a lower mutation rate per locus than VNTRs but deliver more stable and reliable genetic relationships between bacteria isolates, which is more suitable for studies on bacterial phylogenies (Foster et al. 2009).
In comparison, pathway-based approaches that utilize information from multiple loci in a functional unit could produce more stable and robust results than single gene analyses do [7,31].
