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Unexpectedly, these compounds showed a strongly reduced dissociation rate from the K103N mutant, as compared to the wild type enzyme, suggesting that, once occupied by the drug, the mutated binding site could achieve a more stable interaction with these molecules.
The computational docking analysis suggested that the tertiary amine at the meta position of the phenylurea formed a more stable interaction with residues in the back pocket of Aurora-A than in Aurora-B, a possible explanation for the observed discrepancy in the selectivity.
On the other hand, under this condition, UBF was co-precipitated with RPA194 and RPA116, suggesting a more stable interaction.
In addition to all the stable hydrogen bonds formed at these interfaces in the apo form, one more stable interaction was observed at the interface TM2-TM11 in substrate bound form.
There are two cases GcvB-STM43511 and MicC-ompC) where each of those three methods predicts the same energetically more stable interaction than the annotated one.
In the signaling subdomain, we suggest that a larger or more stable interaction with CheA and/or CheW reduces exchange at long times.
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The clones with the more stable interactions are easily found as colonies with the larger blue halos, due to the increased extracellular LacA3 expression.
This anti-diffusive behavior, along with the tonotopic variations in frequency, facilitates unlimited dephasing within synchronized chains, and thus the eventual dissolution of any such groups as their extreme members form more stable interactions with the adjacent groups.
The same study also found that the elimination of a disulphide in a homologue need not always result in more stable interactions between equivalent residues, and about 35% of the poorly conserved disulphides show gaps in their alignment [7].
Recruitiment of Tyr427' into αM' results in improved helical geometry and more stable interactions between αM' and other connection' residues.
Watson−Crick pairs provide more stable interactions, but if one presumes the expected base pairing and number of hydrogen bonds, then the difference in stabilities is not explained.
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