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A lower M value indicates more stable expression.
Through electrotransfer into the ApoE−/− mice hindlimb ischemic muscle model, we show that the IRES-based vector gives more stable expression than either monocistronic plasmid.
The present study on C. batrachus revealed novel candidate RGs (RPL30, TUB, 28S rRNA and ELF-1A) with a more stable expression in experimental contexts, in comparison to frequently used RGs (e.g. ACTB, GAPDH and 18S).
This may result from the more stable expression of transgenic E2F3a protein in this system as compared to E2F2 (Figures 2 and 3), and suggests that E2F3a and E2F2 may be differentially subject to post-translation control mechanisms [6].
Our results clearly reveal novel candidate housekeeping genes with a more stable expression in different cellular and experimental contexts in comparison to frequently used housekeeping genes (e.g. ACTB, GAPDH and HPRT).
The more stable expression of foreign antigens in mycobacteria using integrating vectors as opposed to episomal vectors has been extensively documented [56], [57], [58], [59] and is mainly attributed to lower expression, and therefore a lower metabolic burden, associated with the reduction in the copy number.
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As a whole our results are consistent with a hypothesis that strong gene body methylation leads to more stable gene expression while weak methylation facilitates flexible expression, although the direction of causality remains to be confirmed.
A more numerically stable expression for the mirror-center normal is then presented that is more useful than the original mirror normal expression in the tracking angle formula.
These results may prove useful for the design of more stable retroviral expression cassettes able to counteract chromosomal position effects.
The more stable the expression of a gene is, the lower is its M value.
This finding again confirms that incorporation of network information may lead to identification of more stable gene expression signatures.
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