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In contrast, in GFP-lamin A transfected cultures (SW480/lamA), cells had a more spindle like appearance and grew as a monolayer at low cell density (Figure 4A).
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CD24+ cells displayed a more spindle-like cytoplasm.
PRRX1-expressing cells showed a more spindle-like shape than did mock cells.
In the presence of fiber extract NHEK cells appeared longer and more spindle-like, with more protrusions.
MDA-MB-231 cells displayed more spindle-like morphologies on nanofiber where the elongations followed the underlying topography.
Tumor cells in which SNAI2/Slug was overexpressed displayed a more spindle-like morphology when compared to control cells.
The epithelial MCF-7 and T47D cells cultured with CAF-CM showed more spindle-like shape and cell scattering.
The epithelial cytokeratin-based intermediate filament network is replaced with vimentin (VIM) along with actin (ACTA1) stress fiber formation, yielding a more spindle-like shape in vitro [ 11].
Prdx1−/−;H−Rappeareds alsoared also more spindle-like than Prdx1+/+;H−RasV12MEFs, whereas expression of Prdx1WT in Prdx1−/−;H−RasV12MEFs reversed the spindle-like morphology.
During the culture of these cells, we noticed a change in cell morphology from the cobble stone-like shape typical of epithelial cells to a more spindle-like and scattered appearance, indicating these cells may be undergoing EMT.
As recently described (Recktenwald et al, 2007), the morphology of Kras transfectants was characterised by a more spindle-like shape and appeared less flat as parental and mock control cells.
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