Sentence examples for more specific primers from inspiring English sources

This was not investigated further, but more specific primers better suited to discriminate vertebrate from tsetse DNA will need to be designed.

The most sensitive method to date, loop-mediated isothermal amplification (LAMP) [7], relies on amplification of a multicopy transposon-like sequence for detection of T. brucei, with more specific primers for subspecies determination.

More specific primers towards these 11 sequences should be designed to dissect this.

To identify remaining Wnt orthologues a combination of more specific primers were used (Additional file 2).

These sequences were aligned with relevant cp-regions of confirmed related cp-genomes from Genbank to design more specific primers.

More specific primers were designed for PCR amplifications to bridge two remaining gaps (primers available on request).

From these sequences we designed a more specific primer (5S-Poll-R2) which differs by only 2 nt from 5S-Poll-R.

Multiple bands are not common in the Phi class, probably thanks to more reliable gene structures inferred by the computational analysis and, as a consequence, to a more specific primer design.

If any members of PG1-10 was not initially retrieved with the general primers, e.g., HoxC5 of PG5 could not been found, a PG specific forward primer HoxF5S would be used; if the PG specific primers still failed to amplify the gene, a more specific primer HoxFC5 would be used to confirm its presence or absence.

The partial ORF1b sequences generated with the AstV broadly reactive ORF1b primers were used to design more specific primer sets and probes for reverse transcription PCR and RT real-time quantitative PCR (RT-qPCR) able to identify and quantify the rabbit AstVs (Table 2).

Further investigation of these taxa using more recently collected specimens and more specific PCR primers is currently underway.