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Because drugs that disturb the microtubule network show high general toxicity, we speculated that a more specific interference with lysosome trafficking could result in anti-cancer strategies with fewer side effects.
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We have employed two strategies to inhibit GSK-3α and β function: the use of selective chemical compounds, which were already extensively characterized, as well as the more specific RNA interference technology.
In the second part, we show more specific properties for interference functions based on a nonnegative coupling matrix.
Peake et al. [ 29] indicated that the enzymatic creatinine assay, although theoretically more specific, can have interference problems.
Therefore, exploratory research on the CD28 molecule has attempted to generate more specific effects, avoiding interference with the closely related CTLA-4.
Today, more specific methods such as RNA interference (RNAi) are used to interfere with a given process.
Since these Smad2 phosphorylation inhibitors are also known to block p38 phosphorylation, the role of Smad2 was further explored by the more specific genetic approach of RNA interference (siRNA).
This suggests interferences of more specific proteases than those solely depending on the coagulation and complement activation.
Further studies using more specific inhibition techniques (for example, RNA interference) will be required to conclusively identify the NOS isozymes involved in different lineages.
This can consist of six consecutive tiles, or a more dispersed allocation for robustness against subcarrier specific interference.
The other studies used more specific high-performance liquid chromatography assays where interference does not occur (Fischer et al. 2003).
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