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Of course, studies focusing on finding more specific enzymes are also needed.
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To address this issue, we tried employing the more specific enzyme trypsin, rather than PK, to distinguish PrPC from PrPSc.
Given the nonspecific nature of PK digestion, we chose to digest the samples with a more specific enzyme, trypsin, which cleaves only on the C-terminal side of lysine and arginine residues.
SGPT is more liver specific enzyme.
Combining the complete enzymes set of two fungi is likely to improve saccharification efficiency more than adding specific enzymes to the cocktail produced by a single species.
We found that lyses in a SDS/β-mercaptoethanol buffer and exposure to microwaves is as well suited for detecting fewer than 10 microor-ganisms per sample as a longer, more elaborate and more expensive method using specific enzymes.
In conclusion, the trend in this field is to make enzymes more specific, stable and efficient, and easily controlled for foods production.
Additional in vivo studies will help to elucidate this issue, and also further efforts should be directed to find new PIs that are more specific for these parasite enzymes.
This study may be useful in the development of potent and more specific inhibitors of the enzyme.
Enzymes catalyse more specific reactions inside living systems under mild conditions; therefore, they are efficient alternate for chemical catalysts [1].
The enzyme may be more specific to peptidoglycans of Gram-negatives as the enzyme can lyse only Enterococcus sp. among eight Gram-positive bacteria tested.
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