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He suggested that this would be a warning sign of a more significant sequence of events to come.
In an effort to isolate the virus for more significant sequence information, we randomly selected eight pools, including those that were positive with only the Nphlebo2 primer set, and seeded them onto Vero cells to grow the virus.
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The sequences for some of the more significant sequences are shown in Table 2.
We anticipate that this dataset will be expanded and refined further as more significant transcriptome sequencing efforts are conducted in other Euphorbiaceae species.
As a result, though SAM returned approximately 20% more statistically significant sequences than our method in the WT model; the vast majority of them (85%) did not satisfy a fold change cutoff of 1.25.
This strategy is advantageous over rational engineering approaches in that it requires minimal knowledge of the metabolic pathway, and because selective pressure is being used, a large library of mutants can be generated to cover a much more significant portion of sequence space to generate the desired phenotype.
It has been noticed that the bit rate reduction is more significant for the sequences with higher motion activity.
Similar to the encoding application, the bit rate reduction is more significant for the sequences with higher motion activity or higher resolution.
In the case of Rubus spp., where a reference genome is not yet available, the lack of key informatics poses an even more significant barrier to sequence-based SNP assays, such as the inability to align the SNPs to a reference, which requires additional work to assemble the sequencing reads.
Despite sequencing depth being an important consideration in RNA-seq, it has been shown that the number of biological replicates is a more significant factor than sequencing depth, or technical replicates, in the accurate detection of differentially expressed genes [ 18, 23, 24].
Only 56 sequences from these libraries were found to have a more significant similarity with fungal sequences than with plant sequences (Table 3).
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