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More severe pretreatment conditions generally produce pretreated solids that are easily saccharified by enzymes, but such conditions often cause greater sugar degradation losses and require costly detoxification steps, often referred to as conditioning.
Assuming limited accessibility to cellulose to be the main obstacle for enzymatic hydrolysis [ 30], the effect could tentatively be attributed to improved enzyme accessibility caused by the more severe pretreatment producing a larger surface area by altering the physical shape of the pretreated material, i.e. by creating a more favorable topology.
However, at more severe pretreatment conditions, the concentration of the inhibitory compounds that limit the enzymatic hydrolysis would be higher.
Effective removal of inhibitory compounds would allow the use of more severe pretreatment conditions, which can improve sugar yields and lead to more efficient fermentation.
However, microfibrillated cellulose was not detected on the barks surface, suggesting the need for more severe pretreatment conditions to obtain pure cellulose fibers.
These results come from experiments comparing the impact of milder pretreatment conditions on the transgenic COMT down-regulated and wild-type switchgrass variety 'Alamo', used previously with more severe pretreatment [ 7], in conjunction with fermentation by C. thermocellum.
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A possible explanation for this is that more severe pretreatments (that is, at higher H2O2 loading) are more effective at dissociating xylan from cellulose, and thus less xylanase is required to facilitate access of the cellulases to the cellulose [ 31, 35].
The more severe the pretreatment, the more low molecular weight (DP < 9) xylo-oligomers relative to high molecular weight xylo-oligomers are detected in the reaction media for both DA and hydrothermal pretreatment.
Although, it appears that bark might require more severe steam pretreatment to overcome its inherent recalcitrance, the possible unfavorable structural changes in the steam-pretreated bark could also hamper the enzymatic hydrolysis.
The more severe steam pretreatment at higher temperatures likely depolymerised the extractives, resulting in their dissolution and reduced presence in the insoluble biomass and thus not contributing to the lignin quantification.
Several process parameters can be listed for enhancement of the overall lactic acid yield and productivity such as improving the accessibility of polysaccharides by a more severe lime pretreatment, enzyme dosage, type of enzymes, B. coagulans strain, size and growth phase of inoculum, pH gradient in SSF, and in situ product removal of lactic acid.
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