Sentence examples for more sequencing runs from inspiring English sources

Poretools provides an extensive set of data analysis methods that operate directly on either a single FAST5 file or a set of files from one or more sequencing runs.

Splice junctions that were newly identified from one or more sequencing runs but the same cDNA library were collated and appended to the previous raw junctions prior to a second remapping of reads using TopHat (with all other parameters held constant).

For each amplification method, sequencing reads that mapped to the target genome at 95% or greater accuracy were pooled from three or more individual sequencing runs.

Our observations indicated that GC bias among samples was more substantial between sequencing runs than within runs, suggesting that such bias might have been introduced during the sequencing process, perhaps through slight differences in reagent composition and temperatures that led to differences in DNA denaturation and primer hybridization efficiency.

Using Sanger sequencing, more than 800 sequencing runs (96 capillaries) would be required for equal throughput.

The reliability filter asks whether a signature is present in more than one sequencing run (of the four total runs); signatures observed in more than one run are considered "reliable".

The lower stringency settings on the read quality filters result in 20 50% more useful reads from the sequencing runs for mapping to the reference sequence databases.

This leaves open the possibility of sequencing additional amplicons per sample, or multiplexing more samples per sequencing run in future if necessary.

A total of 106,521 significant (>3 TPM) and reliable (observed in more than one sequencing run) MPSS [ 32] tags were obtained from the Rice MPSS Project [ 32, 76].

Finally, our library preparation and titration procedures produce 2 5 times more reads per sequencing run than standard methods: ~620,000 in our study versus ~130,000 to ~390,000 in previous reports [ 17, 21- 23].

A mere 1 μL of the ligated and reverse transcribed cDNA pool is used during library pre-amplification in a 100 μL reaction volume, which generally yields more than enough library for several sequencing runs.