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The underlying problem is unlikely to be solved by more sequencing: for example, the breast-cancer-associated genes BRCA1 and BRCA2 have been sequenced in over a million patients and yet a high proportion of returned results continue to be VUS.
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This approach eliminates far more sequence for each HCV genome than is actually under immune selection in a given patient, but its advantage is that the remaining sequences are very unlikely to be under substantial immune selection.
Addition of more sequences for different cyclostome lineages (e.g., southern hemisphere lampreys or hagfish) might also provide more evidence.
Alignment algorithms find optimum alignments and maximum alignment scores S of two or more sequences for a given scoring system.
These newer technologies hold the promise of not only generating more sequence for less money, but they may eventually eliminate the concept of draft microbial genomes all together.
Although this study has relatively few sequence loci available for comparison, our findings suggest that a denser RAD marker scan, using a more frequently cutting restriction enzyme would interrogate more genome sequence and interrogate more sequence for comparative analyses.
The reliability of family association for these sequences need to be verified further and the representative profiles need to be enriched with more sequences for genuine cases of family memberships.
With the growing number of genome sequences, we can expect to get more sequences for obligate intracellular and free-living bacteria in all phyla, in order to make comparative genomic analyses including phyla remaining unstudied to date.
Much more sequencing is necessary for WGS.
So this makes intron signal irrelevant except for the requirement for more sequencing depth.
More sequence information for these North American viruses, particularly for the H gene, might give further insight into their origin and evolution.
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