One prerequisite is the availability of an entire genome sequence for the species under consideration, a condition that is increasingly more relaxed with more genome sequences becoming available.
With more plant genome sequences becoming available, it should soon be possible to more precisely address the intriguing questions concerning the extent and evolution of AS in plants.
Essentially, instead of gene sequences becoming progressively more dissimilar, the sequences remain highly similar or even identical.
Yet, these transcripts represent 93% of the gene families identified in Trichomonas [ 57], indicating that (a) sequencing depth appears to be sufficient and that the numbers are not likely to change much with more sequencing data becoming available, and that (b) most of the functional proteome the genome encodes is expressed, but not all members of a gene family.
With whole-genome sequencing becoming more affordable and paired-end technology now available, flow-sorting of derivative chromosomes becomes less critical, as essentially, pairs of reads mapping to different chromosomes will identify translocations.
This is certainly an area that will become more accessible with whole genome sequencing becoming cheaper though the associated computational challenges will be even greater.
With sequencing becoming ever more affordable and accurate [ 31] we will be able to sequence the mitochondrial maxicircle from representatives of each DTU and re-evaluate the three-clade scheme to redefine or further confirm the population structure as well as uncover markers for strains and clades to better understand Chagas disease.
With next-generation DNA sequencing becoming cheaper and more powerful almost monthly, it should soon be possible to sequence whole-plastid genomes for scores of species.
With the ever-increasing development of genome sequencing technologies, more and more sequence information is becoming available to analyze and annotate.
As more genome sequences are becoming available, we are noticing the emergence of underexplored or neglected organisms as alternative resources for new therapeutic agents.
