These platforms are able to generate more sequence data and are substantially less expensive than the original "capillary" Sanger methods.
Further development of the All-Pinus gene database is also valuable as more sequence data from related species in the Pinaceae becomes available.
Further, the characteristic residue list can be refined with the availability of more sequence data in the future.
Because the divergence order of these lineages has remained contentious, more sequence data and data from additional taxa are necessary.
Although the available EST libraries for L. bicolor contain approximately 20K clones, the amount of sequence data from the single transcriptomic data set generates 30-times more sequence data than the combined EST reads.
The resulting trees (Figure S1A, B) are not fully resolved indicated by bootstrap values below 95%, but do not contradict other reconstructions of primate phylogeny that use much more sequence data [21].
To better understand the KTT evolution, people need more sequence data of DNA and protein of KTT superfamilies from different venomous animals and more information of the structure-function relationship of KTTs with different functions.
It would be surprising if more sequence data as provided by the three loci together would have resulted in weaker resolution or lower statistical support for various topologies than a single locus alone.
More sequence data might resolve this issue in the future.
Therefore, more sequence data leads to an increase in precision.
More sequence data are necessary to confirm these findings.
