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To obtain more sequence coverage, TRP47 was digested with Asp-N endopeptidase resulting in 23 peptide fragments.
Therefore, more sequence coverage would be expected to yield a more complete genome.
Some lines provided more sequence coverage than others, with the relative contribution of each noted in Table S1.
The greater the number of segregating alleles, the more sequence coverage is needed to accurate identify each allele and each genotype at a site.
Indeed, we found that species with multiple individuals in the sample had more sequence coverage than those of similar size, but with a single individual.
Because only ∼60% of the three BAC libraries (134,182/223,640) were used to construct the FPC map, there were more options with the larger pools to select clones that had more sequence coverage and less overlap with adjacent clones.
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However, low-level DNA produced much more varied sequence coverage and in forensic use the Ion PGM™ system will require careful calibration of the total samples loaded per chip to preserve the genotyping reliability seen in routine forensic DNA.
Fragmentation of RNA and/or cDNA has been used previously [6] to achieve more even sequence coverage throughout the length of transcripts.
Due to current technical restraints in SMRT methylome analysis, only m6A residues could be detected, as identification of m5C residues requires more extensive sequence coverage.
The SMRT technology also produces more even sequence coverage of the high mol% G + C DNA found in actinomycete genomes than other NGS platforms [ 8].
Notably, more complete sequence coverage was obtained by using ETD compared to CID for these peptides (see Supplemental Figure S1A,B, Supporting Information).
More suggestions(15)
more sequence information
more sequence skill
more sequence diversity
more sequence divergence
more sequence design
more shutdown coverage
more event coverage
more sequence identity
more campaign coverage
more hurricane coverage
more sequence variability
more terrorism coverage
more cult coverage
more sequence downstream
enhanced sequence coverage
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