However, these techniques have important biological cost requiring long periods of treatment and are more sensitive to technical errors [2, 3].
We detect an increasing number of compounds as technical methods become more sensitive, so finding structure among the two dozen or more compounds detected has often relied on statistical analyses using multivariate methods such as Principal Components Analysis[24].
In addition, the putative protective effects of amino acids at physiological concentrations on mitochondrial function – whether alone or in combination with ketone bodies – deserves further study in the light of recent technical developments including more sensitive means of measuring oxygen consumption and ROS generation.
Therefore, single-cell proteomic studies have the technical challenge of developing more sensitive methods for detection.
However, an option for increasing quality of quantification in case of low amount of sample and missing replicated samples is to divide one sample into technical replicates and use more sensitive approaches like 2D fluorescent difference gel electrophoresis (2D-DIGE).
The new IDH1/2 PCR assay has a high technical success rate and is more sensitive than Sanger sequencing.
For technical reasons, the technique would be more sensitive on Tevatron data than on LHC data, they explained, because the Tevatron collided protons and antiprotons, whereas the LHC collided protons and protons.
First, technical advances in electronics and signal processing allow much more sensitive searches.
In order to isolate more candidate genes, several technical improvements in the screens may be required, including more sensitive assay systems (e.g. use of reporter animals), RNAi-based screens using rrf-3 mutants, or soaking RNAi-mediated screens.
Along with the tremendous technical progress in the field of MS-based proteomic studies, more sensitive and specific HDL modifications will be discovered and quantified.
