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Recently, cytokine specific ELISPOT assays or tetrameric MHC-I/ peptide complexes have utilized to directly quantitate circulating CD8+ effector cells, and these assays are more sensitive, quantitative and reproducible than the traditional CTL lysis assay and can also be performed on cryopreserved cells.
The more sensitive quantitative assays of transcription are those based on solution hybridization, such as in nuclease protection and the various microarray and PCR-based assays.
To confirm this data, we also used more sensitive quantitative ELISA method and demonstrated that the levels of soluble tau were unaffected by DNA vaccination (Figure 7B).
We chose to follow up the Northern analysis above with the far more sensitive quantitative RT-PCR analysis and to combine this with an Actinomycin D assay in order to evaluate stability of the messages over time.
To explore potential differences in ZEB1 using the more sensitive quantitative real-time polymerase chain reaction (RT-PCR) method, we analyzed the mRNA expression of ZEB1 and its target IL2 in lymph nodes collected at day 7 p.i. from MOG-immunized DA and PVG.1AV1 rats (N = 6 per group).
Since the introduction of molecular-based techniques, more sensitive quantitative methods have been developed, for instance, based on PCR methodology.
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Therefore, a more sensitive and quantitative assay is required to determine the BteA-dependent cytotoxicity of B. pertussis.
We next reduced the levels of QKI expression in Schwann/DRGN cultures using the same strategy except we used a more sensitive assay, quantitative RT-PCR, to monitor knockdown in rat Schwann cells [39].
Given the hypothesized importance of MOI in regulating DC function and ability to promote robust CD8 T cell responses [7], [8], [9], [10], we assessed DC infection by a more sensitive and quantitative approach.
To answer the question whether the effect of ATP is the result of direct quenching of the energy transfer step or whether it is affecting the fluorescence characteristics of either one or both of the fluorescent moieties in the FRET sensor, we employed a more sensitive and quantitative method; time-resolved fluorescence spectroscopy [8].
Most fluorescent dyes are more sensitive than quantitative visible stains (Table 2).
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