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With this aim, and in view to manage a small quantity of RNAs, we decide to use a more sensitive procedure to detect the miRs (taqMan-microRNA assay; Thermo Fisher, US).
As a more sensitive procedure, HMM could overestimate the functional gene numbers without manual curation.
The expression of UBB+1 in brain sections of UBB+1 tg mice was examined using the UBB+1-specific Ubi3 antibody (bleeding 16-9-97) and a more sensitive procedure (avidin biotin method) than previously used [ 11].
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Probably, the use of more sensitive procedures such as PCR, currently available, would have increased these frequencies.
Functional IRES elements have been proposed in several eukaryotic genes, but subsequent studies using more sensitive procedures have questioned the validity of several of them [ 29- 31].
Thus, it is conceivable that effects of post-learning sleep on the generation of false memories could be revealed with more sensitive testing procedures.
The method proposed for measuring the fidelity of cDNA preparations is intrinsically much more sensitive than procedures that measure the integrity of 16S rRNA.
This is mostly due to a better and earlier recognition of disease, and to more sensitive diagnostic procedures [ 12].
15 One way to reduce the required sample size would be to study a population at high risk of pneumococcal pneumonia using more sensitive diagnostic procedures than blood culture.
The method is faster and five times more sensitive than procedures recommended previously: limit of detection and limit of quantification are 5 and 16 ng/mL, respectively [modified (Ferretti et al. in J Chromatogr B 710 157 164, 1998): 20 and 60 ng/mL].
However, the PANSS is not a scale designed to quantify drug withdrawal effects, and a more sensitive scale or procedure may have shown typical nicotine withdrawal effects.
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