Sentence examples for more sensitive nested from inspiring English sources

The PCR reaction conditions were optimized to permit detection of the organism from agar plates, broth culture, frozen samples, dead fish tissue, and live fish in less than 5 h (8 h, if the more sensitive nested PCR is used).

Although multiplex PCR is the current standard for bacterial meningitis surveillance in the meningitis belt, the more sensitive nested PCR technique would provide more accurate information for evaluation of a new diagnostic test.

Comparative analysis of the two PCR techniques (data not shown) revealed that of 118 samples positive for N. Meningitidis serogroup A by the more sensitive nested PCR technique, only 37 (31%) were positive by multiplex PCR (there were no additional samples positive by multiplex PCR, i.e. all samples negative by the nested technique were also negative by multiplex).

Viral genomes were screened in 70 bone marrow samples from ALL patients through standard and a more sensitive nested PCR.

Positive samples were detected only through a more sensitive nested PCR, most likely reflecting normal levels of infection according to the age of the tested population.

P. malaria, or P. vivax by using either the single-round PCR protocol of Padley et al. (7 ) or the more sensitive nested protocol of Snounou et al. (8 ).

Using a DNA template extracted from PemoNPV-infected shrimp by a viral nucleic acid kit, the detection limit of LAMP was 0.7 fg while that of nested PCR was 70 fg; therefore, the LAMP assay was 100 times more sensitive than nested PCR.

The assay was shown to be 10-fold more sensitive than nested PCR and was able to detect concentrations of 10−9 (approximately 0.01 pg/μL).

These data seem to suggest that real time PCR could be more sensitive than nested PCR.

The results showed that our real-time RT-PCR assay was the most sensitive of the 3 assays, 10-fold more sensitive than nested RT-PCR (Table 2).

In this study, we demonstrated that our new multiplex real-time RT-PCR assay that specifically targets 2 different regions of the H5 gene is more sensitive than nested RT-PCR and even more sensitive than real-time RT-PCR with a single set of primers and probes (unpub. data).