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Interestingly, the existence of valine moieties in the Val-GQDs results in a more sensitive fluorescent response to Hg2 +.
For the fibre tip sensor with the fluorophore functionalized onto the fibre tip using a silica sol-gel film, it was found that higher Rhod-5N concentrations result in a more sensitive fluorescent response but a higher detection limit of 25 ppb in filtered river water.
Moreover, the present study used the more sensitive fluorescent technique to define a microscopy-positive sample; the incremental yield of culture should be even greater in settings that do only light microscopy.
With the development of more sensitive fluorescent and bioluminescent reporters and sophisticated imaging equipment, it has become possible to interrogate the spatiotemporal architecture of the circadian cycles of gene expression in the SCN.
The originally applied DNA technique was a nested PCR [ 114] and was subsequently replaced by 1000 times more sensitive fluorescent PCR, which also reduced the rate of allelic drop out (failure to amplify one or two alleles in a heterozygous cell) from 21%to5.2%2%, enabling a much smaller loss of embryos due to misdiagnosis [ 119].
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For the in vivo experiments, we selected as marker the Luciferase reporter protein, since bioluminescent Luciferase imaging proved to be more sensitive than fluorescent GFP imaging.
Luciferases are presently more sensitive than fluorescent reporters, thanks to a high quantum yield associated to a very low background level of luminescence [ 12, 13].
Although chemiluminescence is more sensitive than fluorescence in nearly all systems, fluorescent reporters still have some advantages; for example, fluorescent proteins were suited to bioimaging assays in living cells which does not require cell permeabilization or the addition of exogenous substrates [ 18].
Nevertheless, the more sensitive IVIS200 fluorescence camera did demonstrate a faint fluorescent signal in both nodes ex vivo.
The qPCR assays were more sensitive than the indirect fluorescent antibody test (IFAT) and the reverse-line blot (RLB) during the early onset of the disease.
Bioluminescent assays with this compound with H1N1 and H5N1 neuraminidases were approximately 20- and 16-fold more sensitive, respectively, than the fluorescent method with the commercial substrate 4-MUNANA.
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