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The trimmed reads were mapped to miRNA precursor sequences in miRBase 16.0 [ 31] to achieve more sensitive expression profiles using the software miRExpress [ 32].
In the majority of cases there was a good correlation between RT-PCR and microarray results, RT-PCR being more sensitive; expression ratios were generally underestimated by microarray analysis.
More sensitive expression data from NGS and RT/q-PCR could be added in the upcoming versions of p-TAREF, depending upon the kind of experiments performed on these platforms and their public availability.
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If d N is more sensitive to expression level than is d S, d S/d N will decrease with expression level, as it does in figure 8.
Inherent to this method, adding biological replicates results in more accurate variance estimation, and therefore in more sensitive differential expression statistics, typically reflected in higher proportion of differentially expressed genes when having more samples.
To gain a more comprehensive and system-wide understanding of molecular pathways in GBM, more sensitive gene expression profiling technology is needed.
This two-step process allows amplification of the resulting signal which, in theory, should give rise to a more "sensitive" transient expression assay system for detecting oestrogen chemicals than has been developed previously.
These more sensitive co-expression analyses emphasize differences in gene network up- or downregulation rather than individual gene expression differences [ 46].
Recently, high-density Affymetrix array data for rice has become publicly available, thereby enabling more sensitive co-expression profiling analysis for rice [20].
The dimers of AP-1 bound with MMP9 promoter DNA are greater than that of MMP2 [ 40, 42], hence regulation of AP-1 due to MMP9 expression is more sensitive than through MMP2 expression.
Musically experienced listeners (with >6 yrs. of musical training) were more sensitive to performance expression than less experienced listeners.
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