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Most novel miRNAs were not detectable by northern blotting, except Can_miR 10, but all were confirmed by using more sensitive array analysis (see next section).
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This principle should allow the team to build more sensitive arrays.
Although moderate-to-good agreement has been reported between the two methods, validation of DNA microarray results by the more sensitive PCR array is generally recommended [38].
The second SWIR hyperspectral camera is similar, although a more sensitive InGaAs array (640HSX-1.7RT, Sensors Unlimited, NJ) was used along with a spectrometer having 3.4 nm sampling, giving 209 spectral bands from 967 to 1680 nm.
For low-density GaAs DE QDs, easier handling of them has huge merit in a silicon-based industry, since GaAs DE QDs as a single photon source with an emission of approximately 700 nm could be applied to low-cost and more sensitive silicon array detectors.
Initial testing had shown that even the more sensitive Gene Array 350 Kit (see below) ideally requires 5 μg of total RNA per sample and experiment.
We used the more sensitive liquichip array system for detection of globin gene mutation and found 23 types of globin gene mutations.
The qRT-PCR based platforms promise to be more sensitive than array based miRNA quantification platforms [ 12], and their use for analyzing samples with low miRNA levels, such as human plasma, is increasing [ 13- 18].
Our findings confirm previous results, but as this method is more sensitive than array based approaches, we identified an extended repertoire of genes modulated by oxygen in an FNR-dependent or -independent manner.
The use of oligonucleotide-array CGH (aCGH) provides a much more sensitive assay for the detection of genomic variance than traditional CGH methods.
Besides the breast, lesions located in other anatomic sites such as the skin, arm or leg, head and neck, and lymph nodes may also be detected with the next generation of PAT devices equipped with more powerful 1064 nm lasers and a more sensitive ultrasonic detection array.
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