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These problems could be solved, at least in part, by using suitable antigens and developing more sensitive antibody detection techniques [ 17, 18].
Our method of detection is more sensitive (antibody based immunofluorescence vs. culture), but this difference in frequency of detection may also be due to geographic location or the fact that our study occurred almost 20 years after the reports referenced.
A more sensitive antibody may reveal low levels of MGL in other spinal regions in the future, but this characteristic immunostaining pattern apparently predicts that the majority of the MGL enzyme is highly concentrated in neuronal and/or glial elements of the nociceptive circuitry in the superficial laminae of the dorsal horn.
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We also observe a positive correlation between the skewness and inter-profile reproducibility (Additional file 2: Figure S7), suggesting more sensitive antibodies may produce more consistent profiles between the two technologies.
In our experience, the occurrence of such cells has become a more frequent diagnostic problem in recent years, presumably reflecting the utilization of more sensitive antibodies and immunohistochemical techniques.
There is emerging evidence that at least some culture-adaptive mutations render the strains more sensitive to antibody neutralization (Dhillon et al., 2010; Grove et al., 2008).
Recent studies have shown that CD4+ T cells infected with HIV-1 mutants lacking Vpu are more sensitive to antibody-dependent cellular cytoxicity (ADCC) (Arias et al., 2014; Veillette et al., 2014).
Prior to immunization, none of 98 children and 1 of 118 (0.8%) elderly individuals had detectable MN H5-antibody titers while 1/98 (1%) children and 8/118 (6.8%) elderly persons were positive in the H5pp test which is known to be a more sensitive assay for antibody detection [16].
Another study [ 25] among HIV positive MSM showed testing for ALT was more sensitive than HCV antibody testing for early diagnosis of HCV.
It is likely that the loss of cell orientation in advanced neoplasia results in 'spilling' of the normally apically secreted MUC5AC mucin into the circulation, which was then detectable with the more sensitive polyclonal Lum5-1 antibody.
More sensitive detection of antibody to avian HA was seen when horse erythrocytes were used in place of turkey erythrocytes in the HI test because influenza virus was better able to bind to a2,3Gal-specific receptor sites on these erythrocytes (35 ).
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