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The comparison between the MC1R specific mAbs with other melanoma specific mAbs commonly used in melanoma diagnosis showed that although the majority of melanomas were positive for all three mAbs (S-100, HBM45 and MC1R specific mAbs), several tumours expressed a more selective expression pattern lacking one or two of these markers.
In mammals, ANXA1 is ubiquitously expressed, while ANXA3 has more selective expression patterns and tissue distribution [ 24].
Some of the novel microRNAs are expressed ubiquitously (ID 26, 39, 51), while others show more selective expression (ID 46,61).
Among the protein coding mRNAs, the majority is widely expressed, whereas other transcripts, in particular the lncRNAs, display a more selective expression pattern, possibly suggesting distinct functions of a relatively small set of non-coding RNAs required in various brain regions.
This is likely due to a more selective expression of newly-identified microRNAs in different tissues or developmental stages.
It is known that choice of the AAV serotype contributes to better expression of a therapeutic gene and more selective expression in terms of tissue specificity.
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While much attention has previously been focused on the ubiquitously expressed family member MAPK14 (p38α), recent studies indicate that family members such as MAPK13 (p38δ) display a more selective cellular and tissue expression and might therefore represent a specific kinase to target in certain diseases.
To increase certainty that the cells of the vascular wall in the tumor were indeed from the implanted donor orthotopic tissue, we performed additional experiments where the tissue was donated once again from transgenic mice expressing GFP but this time the expression was more selective to cell type because it is controlled by an endothelial cell promoter.
The IC50 values for inhibiting the expression of mutant ATN-1 were similar for REP and P9, 1.2 and 0.7 nM, respectively, demonstrating that duplex P9 was more selective because it better preserved the expression of wild-type ATN-1.
A significant but more selective influence of epigenetic modifiers could also be observed on microRNA expression levels.
Spatio-temporal differences in the expression of processing enzymes may therefore result in a more selective production of inhibitor peptides.
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