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Protein design provides a potential route to generating synthetic variants of native proteins with more selective binding profiles.
This observation may contribute to a better understanding of the more selective binding of o-OCH3 arylpiperazine derivatives to the 5-HT1A receptor.
Concurrently, DIMA increased ER-β, p21 and p27 protein levels in LNCaP cells and exhibited ~ 5 times more selective binding for ER-β than ER-α, in comparison to raloxifene.
The second important window of opportunity is to be found in our ability to expand the set of CBMs to include an even more selective binding capacity and to create a multiplexed assay employing multiple CBMs.
Thus, outside the S phase, unphosphorylated UNG2 was retained more in the chromatin relative to UNG2 p-S23, while in S phase a more selective binding of UNG2 p-S23 was observed.
Construction of chimeric fusion proteins by mixing the A and B domains of various binary toxins to achieve more selective binding of toxin to specific cell types such as tumor cells can be achieved.
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With this threshold, the identified SGTP-binding proteins are at least 5-fold more selective toward binding the modified nucleotide than those proteins that were labeled via nonspecific binding.
Interestingly, recent structural analysis of langerin indicates that it combines a different set of features, in which there are extensive interactions between the CRDs, forming a fixed cluster as in mannose-binding protein, but the individual binding sites are more extended, as in DC-SIGN, leading to more selective ligand binding (Feinberg, Powlesland et al. 2010).
In contrast, new HuEx arrays rely on more selective DNA/DNA binding with significantly different physical properties.
It is conceivable that the more selective ligand-binding profiles of a viral Bcl-2 protein reflect the circumstance that only specific pro-apoptotic Bcl-2 proteins require targeting during viral infection.
This calix[4]crown monolayer showed a more sensitive and selective binding toward potassium ion over other alkali and alkaline earth metal ions.
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more conventional binding
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more miRNA binding
enhanced selective binding
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