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To accurately estimate the magnitude and timing of seasonal forcing, serum samples should be collected every two months and 200 or more samples should be included in each collection; this sample size estimate is sensitive to the antibody waning rate and the assumed level of seasonal forcing.
Future studies with more samples should be needed to confirm this result and elucidate underlying pathogenesis.
More samples should be recruited for our next genome-wide scan.
The analyses of additional DNA sequences from other genes in the Dahongjun genomes as well as the inclusion of more samples should help resolve the issue of additional reproductively isolated subgroups within each of the three lineages.
Only 51 subjects were examined in this study, which was large enough to recognize statistically significant trends, but studies that analyze more samples should be performed.
The numbers of bats submitted in passive surveillance should, however, be higher, and means to obtain more samples should be considered.
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Further investigation should not only comprise larger datasets containing significantly more samples, but should also involve additional high-density platforms, such as Nimblegen.
First, sampling only took place in one university in China, whereas larger and more diverse samples should be used for future investigation.
Larger and more varied samples should be studied to establish the generalizability of our results to patient populations with a variety of occlusions.
In clinical studies that enroll subjects with pre-existing liver chemistry abnormalities, two or more serum samples should be obtained over a one month period in the pre-treatment phase to determine whether the abnormalities are constant or changing.
However, since sample numbers in this study were small, more hepatoblastoma samples should be analyzed to confirm the present data and to evaluate the precise role of these alterations in tumorigenesis in addition to assessing their usefulness as markers for clinical characteristics, such as stage classification, response to chemotherapy, and prognosis.
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