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Due to their high throughput and low time requirements, these platforms are able to analyze more sample volume in a shorter amount of time than traditional detection methods.
The influence of incubation volumes on the capture efficiency of 7.0 × 10 beads was also tested, because in a real-life situation it is possible that more sample volume is required (>100 μL) to capture the minimum amount of OTA which is detectable by the LC-MS.
In shake flask and micro-bioreactor experiments, dry cell weight (DCW) is also determined due to the availability of more sample volume (~5 mL), so that the total lipid content of each sample (total lipid/DCW) is determined to better evaluate the biomass, lipid, and EPA productivities.
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With regard to the factor interactions, more sample amount, higher extractant volume, and longer rinsing time were necessary to achieve the best recoveries (see Fig. 2).
This phenomenon may explain why the relative efficiencies of 18F, 11C, and also 125I are not more strongly dependent on sample volume when counted on a lower energy photopeak window.
New instrumentation (i.e., the PCR rapid assays) was brought into BTRL to attempt to process the sample volume more quickly.
A commercial 5 mm C optimized probe would provide about 7-fold greater absolute sensitivity with 15-fold more sample than our small volume probe.
Therefore, cDNA prepared from RNA extracted with protocol 1 might had slightly more amplifiable templates per sample volume than cDNA prepared from total RNA extracted with protocol 2. The results from the multiplex endpoint RT-PCR assay were validated by the results obtained from the RT-qPCR assay and allowed the identification of FFPE samples with an adequate RNA quality.
A flow rate of 8 μl/min was chosen to ensure that no more than 10% of the sample volume was exchanged during the sampling time of a single step-scan experiment of 2.5 ms.
For instance, MS is more sensitive and allows obtaining more detailed information while using small sample volume, but is also quite sensitive to changing experimental conditions, and sample preparation is more demanding.
The comparative analysis has led to the conclusion that the ferromagnetic phase formed in the 18Cr10NiTi steel during deuterium implantation is no more than 2%to3%3% of the sample volume (with due regard for the experimental error).
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