The inherent limitations of arbitrary marker systems in transferability and reproducibility between populations, species and laboratories, however, have meant that, as in the vast majority of plant species, more robust, sequence-characterized marker systems such as microsatellites have gained greater favour in genetic analyses in recent years.
Furthermore, in contrast to microarray, where even a single nucleotide mutation in probe sequence may affect the efficiency of probe hybridization, RNA-seq is more robust to sequence variations between species.
For each consensus, 30 40 copies were isolated from the genome to produce a second and more robust consensus sequence.
Since the protein structure is relatively more robust than sequence during evolution, structure comparison methods can discover remote homologous proteins for a given protein.
When compared to existing methods, GraPPLE was demonstrated to be more robust to sequence divergence between the members of an Rfam family and also exhibited improved prediction accuracy.
We also newly reveal that combinatorial binding is more robust to sequence variations in directly bound motifs and that the more species in which a TF binding region is found, the stronger the genomic occupancy.
With respect to number of contigs/scaffolds, corresponding N50 values and mismatch error frequency, it was found that draft genome generated by MaSuRCA generated a more robust genome sequence compared to the genomes generated by SOAPdenovo2 and CLC bio (Additional file 2: Table S2).
Moreover, the identification of such contamination in non-primate databases would indicate that more robust pre-sequencing pipelines should be established to limit cross contamination among human genome sequences.
Recent development in parallel imaging has renewed the interest in developing more robust TSE sequences for fMRI.
Higher magnetic fields, more intense decoupling power, higher spinning rates, more robust pulse sequences, and novel designs of RF coils remain the routes pursued in achieving better solid-state NMR spectra.
Additionally, the estimated error rate for indel mutations in this data set was lower than the estimated base substitution error rate (Figure S1), as has been shown by others [26], suggesting that indel mutation analysis is more robust to sequencing errors than SNP discovery.
