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Exact(4)
Due to the presence of 11 different oligonucleotide sets for each specific gene being used on the microarray versus only a single primer set for a gene in a quantitative PCR, the microarray is more effective at eliminating false positive or negative data and provides a more robust quantification of changes in gene expression.
This permits the detection of up to 10 cellular divisions and therefore provides a more robust quantification of the proliferation by a cell population than BrdU incorporation (5).
The study of near misses, which occur in far greater numbers than maternal deaths, allows for a more robust quantification and conclusion on the risk factors and determinants of life – threatening complications [ 4, 7].
For a more robust quantification of the effect of PF1052 on neutrophil polarity, we devised a measurement termed the 'polarity index' that reflects the difference in EGFP intensity at the leading edge of the cell compared with the trailing edge, as well as the total fluorescence within the cell.
Similar(55)
Hence, CAPS had a more robust MS quantification that is less sensitive to noise and more likely to identify the major protein variant that will ease ELISA validation.
FRET measurements inside cells might also be combined with single-particle tracking image correlation for following the fate of individual viral complexes, fluorescence lifetime imaging for more robust FRET quantifications, or advanced multicolor fluctuation microscopy for monitoring multiple interactions simultaneously.
Small molecule quantification has several more robust options for quantification compared to proteins, with the benefit that the molecule analyzed is typically intact, and not a digestion product.
Overall this method of qPCR standard preparation achieved a more reliable and robust quantification of 16S rRNA genes.
Previous human studies [11, 12] evaluated the performance of some of these models (plasma input and reference tissue based) and established which ones provided a more robust and reliable quantification of [11C]flumazenil receptor binding.
The more robust and accurate quantification methods use stable isotopes such as C, N and O to introduce a detectable increase in mass.
These experiments establish a more robust technique coupling quantification of Ca2+ radiotracer accumulation in ER microsomes with the hydrolysis and release of P i by ER resident Ca2+-ATPases (SERCAs).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com